What Causes Hyperfluorescence: Folding Intermediates or Conformationally Flexible Native States?
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چکیده
منابع مشابه
What causes hyperfluorescence: folding intermediates or conformationally flexible native states?
Hyperfluorescent intensity maxima during protein unfolding titrations are often taken as a sign for a thermodynamic folding intermediate. Here we explore another possibility: that hyperfluorescence could be the signature of a "pretransition" conformationally loosened native state. To model such native states, we study mutants of a fluorescent ubiquitin variant, placing cavities at various dista...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2002
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(02)75183-7